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Part:BBa_K2047666

Designed by: Yu Jiang   Group: iGEM16_OUC-China   (2016-10-14)


RNase E site

Ribonuclease E (RNase E), which is encoded by an essential Escherichia coli gene known variously as me, ams, and hmp, was discovered initially as an rRNA-processing enzyme but is now known to have a general role in RNA decay. Multiple functions, including the ability to cleave RNA endonucleolytically in AU-rich single-strand regions. This sequence encodes an endoribonuclease cut site that was recognized by RNaseE. Insert this sequence into intergenic region , after being transcribed, the primary transcript will be digested into split segments at this site, thus decouple the upstream and downstream genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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